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| Registros recuperados: 17 | |
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| Renault, Tristan; Moreau, Pierrick; Faury, Nicole; Pepin, Jean-francois; Segarra, Amelie; Webb, Stephen. |
| Although there are a number of ostreid herpesvirus 1 (OsHV-1) variants, it is expected that the true diversity of this virus will be known only after the analysis of significantly more data. To this end, we analyzed 72 OsHV-1 "specimens" collected mainly in France over an 18-year period, from 1993 to 2010. Additional samples were also collected in Ireland, the United States, China, Japan, and New Zealand. Three virus genome regions (open reading frame 4 [ORF4], ORF35, -36, -37, and -38, and ORF42 and -43) were selected for PCR analysis and sequencing. Although ORF4 appeared to be the most polymorphic genome area, distinguishing several genogroups, ORF35, -36, -37, and -38 and ORF42 and -43 also showed variations useful in grouping subpopulations of this... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19445/17221.pdf |
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| Moreau, Pierrick; Moreau, Kevin; Segarra, Amelie; Tourbiez, Delphine; Travers, Marie-agnes; Rubinsztein, David C.; Renault, Tristan. |
| Recent mass mortality outbreaks around the world in Pacific oysters, Crassostrea gigas, have seriously affected the aquaculture economy. Although the causes for these mortality outbreaks appear complex, infectious agents are involved. Two pathogens are associated with mass mortality outbreaks, the virus ostreid herpesvirus 1 (OsHV-1) and the bacterium Vibrio aestuarianus. Here we describe the interactions between these 2 pathogens and autophagy, a conserved intracellular pathway playing a key role in innate immunity. We show for the first time that autophagy pathway is present and functional in Pacific oysters and plays an important role to protect animals from infections. This study contributes to better understand the innate immune system of Pacific... |
| Tipo: Text |
Palavras-chave: Autophagy; Crassostrea gigas; OsHV-1; Pacific oyster; Vibrio aestuarianus; ATG; Autophagy-related; Atg8-PE; Atg8-phosphatidylethenolamine; DNA; Deoxyribonucleic acid; Hpi; Hours postinfection; LC3-II; Cleaved; Lipidated and autophagosome-associated form of LC3; MAP1LC3A; B (LC3A; B); Microtubule-associated proteins 1 light chain 3 alpha. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/37989/36072.pdf |
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| Leprêtre, Maxime; Faury, Nicole; Segarra, Amelie; Claverol, Stéphane; Degremont, Lionel; Palos-ladeiro, Mélissa; Armengaud, Jean; Renault, Tristan; Morga, Benjamin. |
| Massive mortality outbreaks affecting Pacific oysters (Crassostrea gigas) spat/juveniles are often associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted using two contrasted Pacific oyster families for their susceptibility to viral infection. Live oysters were sampled at 12, 26, and 144 h post infection (hpi) to analyze host-pathogen interactions using comparative proteomics. Shotgun proteomics allowed the detection of seven viral proteins in infected oysters, some of them with potential immunomodulatoy functions. Viral proteins were mainly detected in susceptible oysters sampled at 26 hpi, which correlates with the mortality and... |
| Tipo: Text |
Palavras-chave: Proteomics; Interactions; Antiviral response; OsHV-1; Pacific oysters. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00669/78075/80307.pdf |
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| Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan. |
| Background: Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches. Results: The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant... |
| Tipo: Text |
Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf |
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| Segarra, Amelie; Faury, Nicole; Haffner, Philippe; Pepin, Jean-francois; Tourbiez, Delphine; Trancart, Suzanne; Travers, Marie-agnes; Renault, Tristan. |
| Le premier cas d'infection à virus de type herpès a été rapporté en 1972, aux Etats Unis, chez l'huître américaine, Crassostrea virginica (Farley et al, 1972). A partir de 1991, des mortalités massives associées à la détection de virus de type herpes ont été observées chez plusieurs espèces de bivalves marins, à différents stades de développement (essentiellement chez les larves et le naissain) et dans différentes régions du monde. Sur la base d’observation en microscopie électronique à transmission, ces virus présentaient des caractéristiques morphologiques identiques à celles des Herpesvirus (Roizman, 1982). Les connaissances acquises concernant la taille, la structure et le séquençage du génome d’un virus infectant les larves d’huîtres creuses en... |
| Tipo: Text |
Palavras-chave: Herpès virus OsHV-1; Expression gènes; Huître creuse; Crassostrea gigas. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00195/30627/29076.pdf |
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| Renault, Tristan; Tchaleu, Gwenaelle; Faury, Nicole; Moreau, Pierrick; Segarra, Amelie; Barbosa-solomieu, Valerie; Lapegue, Sylvie. |
| Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest.... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00172/28306/26582.pdf |
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| Martenot, Claire; Segarra, Amelie; Baillon, Laury; Faury, Nicole; Houssin, Maryline; Renault, Tristan. |
| Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and... |
| Tipo: Text |
Palavras-chave: OsHV-1; Viral proteins; Crassostrea gigas. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00326/43697/43124.pdf |
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| Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud. |
| Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters... |
| Tipo: Text |
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf |
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| Lafont, Maxime; Petton, Bruno; Vergnes, Agnes; Pauletto, Marianna; Segarra, Amelie; Gourbal, Benjamin; Montagnani, Caroline. |
| In the last decade, a paradigm shift has emerged in comparative immunology. Invertebrates can no longer be considered to be devoid of specific recognition and immune memory. However, we still lack a comprehensive view of these phenomena and their molecular mechanisms across phyla, especially in terms of duration, specificity, and efficiency in a natural context. In this study, we focused on a Lophotrochozoan/virus interaction, as antiviral priming is mostly overlooked in molluscs. Juvenile Crassostrea gigas oysters experience reoccurring mass mortalities events from Ostreid herpes virus 1 with no existing therapeutic treatment. Our results showed that various nucleic acid injections can prime oysters to trigger an antiviral state ultimately protecting them... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00407/51811/52413.pdf |
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| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan. |
| Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf |
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| Segarra, Amelie; Baillon, Laury; Tourbiez, Delphine; Benabdelmouna, Abdellah; Faury, Nicole; Bourgougnon, Nathalie; Renault, Tristan. |
| Since 2008, massive mortality outbreaks associated with OsHV-1 detection have been reported in Crassostrea gigas spat and juveniles in several countries. Nevertheless, adult oysters do not demonstrate mortality in the field related to OsHV-1 detection and were thus assumed to be more resistant to viral infection. Determining how virus and adult oyster interact is a major goal in understanding why mortality events are not reported among adult Pacific oysters. Dual transcriptomics of virus-host interactions were explored by real-time PCR in adult oysters after a virus injection. Thirty-nine viral genes and five host genes including MyD88, IFI44, IkB2, IAP and Gly were measured at 0.5, 10, 26, 72 and 144 hours post infection (hpi). No viral RNA among the 39... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00213/32414/30887.pdf |
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| Segarra, Amelie. |
| Oyster fanning represents a significant economic part of the French aquaculture production. This production is approximately about 150 000 tons of shells a year [Buestel, 2004]. However the development ofthis activity can be limited by infectious diseases (virus, protozoan or bacteria). Flat oysters suffered from two epizooties caused by two parasitic di seases: martieilliosis and the bonamiosis. These diseases appeared at the end of the Sixties and Seventies respectively. These diseases had a negative impact on the economy because they decrease the production of Flat oysters by 90% between 1979 and .1980. [Buestel 2004, Grizel 1985] The means of fights against bonamiosis are limited and mainly rely on a preventive approach. In fact, it is impossible to... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00368/47909/47939.pdf |
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| Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie. |
| Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August.... |
| Tipo: Text |
Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf |
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| Segarra, Amelie; Faury, Nicole; Pepin, Jean-francois; Renault, Tristan. |
| Massive mortality outbreaks have been reported in France since 2008 among Pacific oysters, Crassostrea gigas, with the detection of a particular OsHV-1 variant called μVar. Virus infection can be induced in healthy spat in experimental conditions allowing to better understand the disease process, including viral gene expression. Although gene expression of other herpesviruses has been widely studied, we provide the first study following viral gene expression of OsHV-1 over time. In this context, an in vivo transcriptomic study targeting 39 OsHV-1 genes was carried out during an experimental infection of Pacific oyster spat. For the first time, several OsHV-1 mRNAs were detected by real-time PCR at 0 h, 2 h, 4 h, 18 h, 26 h and 42 h post injection. Several... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; OsHV-1; Viral gene expression; Inhibitors of apoptosis; Real time PCR; Elongation factor. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29549/27875.pdf |
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| Registros recuperados: 17 | |
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